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superscript iii rnase h reverse-transcriptase kit  (Thermo Fisher)


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    Thermo Fisher superscript iii rnase h reverse-transcriptase kit
    Superscript Iii Rnase H Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+rnase+h+reverse+transcriptase+kit/pm38367082-77-5-11
    Average 90 stars, based on 1 article reviews
    superscript iii rnase h reverse-transcriptase kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: Comparison of gene expression profiles of gingival carcinoma Ca9-22 cells and colorectal adenocarcinoma HT-29 cells to identify potentially important mediators of SLPI-induced cell migration.
    Article Snippet: Briefly, total RNA was isolated using RNAiso Plus (Takara Bio, Shiga, Japan), in accordance with the manufacturer’s instructions. .. First-strand cDNA was synthesized from 1.5 μg of total RNA using a SuperScript III RNase H reverse transcriptase kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The cDNA obtained was amplified by performing real-time RT-PCR using a SYBR Premix Ex Taq II (Takara Bio) in CFX96 Real-Time System (Bio-Rad Laboratories, Hercules, CA, USA).

    Article Title: Infectious pancreatic necrosis virus infection of fish cell lines: Preliminary analysis of gene expressions related to extracellular matrix remodeling and immunity.
    Article Snippet: The pathogenic infectious pancreatic necrosis virus (IPNV) causes high economic losses in fish farming.. This virus can modulate several cellular processes during infection, but little is known about the infection mechanism.. To investigate gene activation in response to IPNV, CHSE/F and SHK-1 cell line were infected with a cytopathic Sp field isolate of IPNV, and the expression profiles of proinflammatory, antiviral cytokine, and extracellular matrix markers were analyzed.

    Article Title: Sumoylation of nucleoprotein (NP) mediated by activation of NADPH oxidase complex is a consequence of oxidative cellular stress during infection by Infectious salmon anemia (ISA) virus necessary to viral progeny.
    Article Snippet: To obtain cDNA, total RNA was first extracted from cell pellets with the TRIzol reagent (Invitrogen, USA) following the manufacturer’s instructions, and then treated with DNase I, Amplification grade (1 U/mg RNA; Invitrogen). .. First-strand cDNA was synthesized from total RNA (1 μg) using the SuperScript III RNase H Reverse Transcriptase kit (Invitrogen) with the oligo-dT18 primer at 50 °C for 50 min. Real-time PCR analysis was performed with an ABI PRISM 7500 instrument using the SYBR Green PCR Core Reagents (both from Applied Biosystems, USA). ..

    Article Title: Detection and Molecular Characterization of Canine Alphacoronavirus in Free-Roaming Dogs, Bangladesh
    Article Snippet: .. After extraction of RNA, we synthesized cDNA using the SuperScript III RNase H Reverse transcriptase kit (Invitrogen SA, Spain/Portugal, Barcelona, Spain). ..

    Article Title: Characterization of Reconstructed Human Epidermis in a Chemically-defined, Animal Origin-free Cell Culture System
    Article Snippet: .. Briefly, RNA from RHE was extracted using the “ReliaPrepTM miRNA Cell and Tissue Miniprep System” (Promega Cat#Z6111, Wisconsin, USA) and its concentration measured by NanoDrop 1,000 spectrophotometer (ThermoFisher Scientific, Massachusetts, USA). cDNA was then synthesized using “SuperScript III RNase H-Reverse Transcriptase Kit” (Thermo Fisher Scientific Cat18080093, Massachusetts, USA). .. SYBR Green method (Eurogentec, Liège, Belgium) and specifically designed primers (Eurogentec, Liège, Belgium; Millipore Sigma) listed in were used.

    Article Title: SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role
    Article Snippet: For RT-PCR analysis, total RNA was isolated from cells under standard culture conditions (un-confluent) using RNAiso Plus (Takara Bio, Shiga, Japan) in accordance with the manufacturer’s instructions. .. First-strand cDNA was synthesized from 1 μg of total RNA using a SuperScript III RNase H reverse transcriptase kit (Thermo Fisher Scientific). cDNA was then subjected to real-time RT-PCR using SYBR Premix Ex TaqTM II (Takara Bio) on a CFX96 Real-Time System (Bio-Rad Laboratories, Hercules, CA, USA). ..

    Reverse Transcription:

    Article Title: Comparison of gene expression profiles of gingival carcinoma Ca9-22 cells and colorectal adenocarcinoma HT-29 cells to identify potentially important mediators of SLPI-induced cell migration.
    Article Snippet: Briefly, total RNA was isolated using RNAiso Plus (Takara Bio, Shiga, Japan), in accordance with the manufacturer’s instructions. .. First-strand cDNA was synthesized from 1.5 μg of total RNA using a SuperScript III RNase H reverse transcriptase kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The cDNA obtained was amplified by performing real-time RT-PCR using a SYBR Premix Ex Taq II (Takara Bio) in CFX96 Real-Time System (Bio-Rad Laboratories, Hercules, CA, USA).

    Article Title: Xiaotan Sanjie recipe, a compound Chinese herbal medicine, inhibits gastric cancer metastasis by regulating GnT-V-mediated E-cadherin glycosylation.
    Article Snippet: https://doi.org/10.1016/j.joim.2023.11.001 2095-4964/ 2023 Shanghai Yueyang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine.. All rights reserved.. ⇑ Corresponding authors.

    Article Title: Infectious pancreatic necrosis virus infection of fish cell lines: Preliminary analysis of gene expressions related to extracellular matrix remodeling and immunity.
    Article Snippet: The pathogenic infectious pancreatic necrosis virus (IPNV) causes high economic losses in fish farming.. This virus can modulate several cellular processes during infection, but little is known about the infection mechanism.. To investigate gene activation in response to IPNV, CHSE/F and SHK-1 cell line were infected with a cytopathic Sp field isolate of IPNV, and the expression profiles of proinflammatory, antiviral cytokine, and extracellular matrix markers were analyzed.

    Article Title: Sumoylation of nucleoprotein (NP) mediated by activation of NADPH oxidase complex is a consequence of oxidative cellular stress during infection by Infectious salmon anemia (ISA) virus necessary to viral progeny.
    Article Snippet: To obtain cDNA, total RNA was first extracted from cell pellets with the TRIzol reagent (Invitrogen, USA) following the manufacturer’s instructions, and then treated with DNase I, Amplification grade (1 U/mg RNA; Invitrogen). .. First-strand cDNA was synthesized from total RNA (1 μg) using the SuperScript III RNase H Reverse Transcriptase kit (Invitrogen) with the oligo-dT18 primer at 50 °C for 50 min. Real-time PCR analysis was performed with an ABI PRISM 7500 instrument using the SYBR Green PCR Core Reagents (both from Applied Biosystems, USA). ..

    Article Title: Detection and Molecular Characterization of Canine Alphacoronavirus in Free-Roaming Dogs, Bangladesh
    Article Snippet: .. After extraction of RNA, we synthesized cDNA using the SuperScript III RNase H Reverse transcriptase kit (Invitrogen SA, Spain/Portugal, Barcelona, Spain). ..

    Article Title: SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role
    Article Snippet: For RT-PCR analysis, total RNA was isolated from cells under standard culture conditions (un-confluent) using RNAiso Plus (Takara Bio, Shiga, Japan) in accordance with the manufacturer’s instructions. .. First-strand cDNA was synthesized from 1 μg of total RNA using a SuperScript III RNase H reverse transcriptase kit (Thermo Fisher Scientific). cDNA was then subjected to real-time RT-PCR using SYBR Premix Ex TaqTM II (Takara Bio) on a CFX96 Real-Time System (Bio-Rad Laboratories, Hercules, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: Infectious pancreatic necrosis virus infection of fish cell lines: Preliminary analysis of gene expressions related to extracellular matrix remodeling and immunity.
    Article Snippet: The pathogenic infectious pancreatic necrosis virus (IPNV) causes high economic losses in fish farming.. This virus can modulate several cellular processes during infection, but little is known about the infection mechanism.. To investigate gene activation in response to IPNV, CHSE/F and SHK-1 cell line were infected with a cytopathic Sp field isolate of IPNV, and the expression profiles of proinflammatory, antiviral cytokine, and extracellular matrix markers were analyzed.

    Article Title: Sumoylation of nucleoprotein (NP) mediated by activation of NADPH oxidase complex is a consequence of oxidative cellular stress during infection by Infectious salmon anemia (ISA) virus necessary to viral progeny.
    Article Snippet: To obtain cDNA, total RNA was first extracted from cell pellets with the TRIzol reagent (Invitrogen, USA) following the manufacturer’s instructions, and then treated with DNase I, Amplification grade (1 U/mg RNA; Invitrogen). .. First-strand cDNA was synthesized from total RNA (1 μg) using the SuperScript III RNase H Reverse Transcriptase kit (Invitrogen) with the oligo-dT18 primer at 50 °C for 50 min. Real-time PCR analysis was performed with an ABI PRISM 7500 instrument using the SYBR Green PCR Core Reagents (both from Applied Biosystems, USA). ..

    SYBR Green Assay:

    Article Title: Infectious pancreatic necrosis virus infection of fish cell lines: Preliminary analysis of gene expressions related to extracellular matrix remodeling and immunity.
    Article Snippet: The pathogenic infectious pancreatic necrosis virus (IPNV) causes high economic losses in fish farming.. This virus can modulate several cellular processes during infection, but little is known about the infection mechanism.. To investigate gene activation in response to IPNV, CHSE/F and SHK-1 cell line were infected with a cytopathic Sp field isolate of IPNV, and the expression profiles of proinflammatory, antiviral cytokine, and extracellular matrix markers were analyzed.

    Article Title: Sumoylation of nucleoprotein (NP) mediated by activation of NADPH oxidase complex is a consequence of oxidative cellular stress during infection by Infectious salmon anemia (ISA) virus necessary to viral progeny.
    Article Snippet: To obtain cDNA, total RNA was first extracted from cell pellets with the TRIzol reagent (Invitrogen, USA) following the manufacturer’s instructions, and then treated with DNase I, Amplification grade (1 U/mg RNA; Invitrogen). .. First-strand cDNA was synthesized from total RNA (1 μg) using the SuperScript III RNase H Reverse Transcriptase kit (Invitrogen) with the oligo-dT18 primer at 50 °C for 50 min. Real-time PCR analysis was performed with an ABI PRISM 7500 instrument using the SYBR Green PCR Core Reagents (both from Applied Biosystems, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Sumoylation of nucleoprotein (NP) mediated by activation of NADPH oxidase complex is a consequence of oxidative cellular stress during infection by Infectious salmon anemia (ISA) virus necessary to viral progeny.
    Article Snippet: To obtain cDNA, total RNA was first extracted from cell pellets with the TRIzol reagent (Invitrogen, USA) following the manufacturer’s instructions, and then treated with DNase I, Amplification grade (1 U/mg RNA; Invitrogen). .. First-strand cDNA was synthesized from total RNA (1 μg) using the SuperScript III RNase H Reverse Transcriptase kit (Invitrogen) with the oligo-dT18 primer at 50 °C for 50 min. Real-time PCR analysis was performed with an ABI PRISM 7500 instrument using the SYBR Green PCR Core Reagents (both from Applied Biosystems, USA). ..

    Extraction:

    Article Title: Detection and Molecular Characterization of Canine Alphacoronavirus in Free-Roaming Dogs, Bangladesh
    Article Snippet: .. After extraction of RNA, we synthesized cDNA using the SuperScript III RNase H Reverse transcriptase kit (Invitrogen SA, Spain/Portugal, Barcelona, Spain). ..

    Concentration Assay:

    Article Title: Characterization of Reconstructed Human Epidermis in a Chemically-defined, Animal Origin-free Cell Culture System
    Article Snippet: .. Briefly, RNA from RHE was extracted using the “ReliaPrepTM miRNA Cell and Tissue Miniprep System” (Promega Cat#Z6111, Wisconsin, USA) and its concentration measured by NanoDrop 1,000 spectrophotometer (ThermoFisher Scientific, Massachusetts, USA). cDNA was then synthesized using “SuperScript III RNase H-Reverse Transcriptase Kit” (Thermo Fisher Scientific Cat18080093, Massachusetts, USA). .. SYBR Green method (Eurogentec, Liège, Belgium) and specifically designed primers (Eurogentec, Liège, Belgium; Millipore Sigma) listed in were used.

    Spectrophotometry:

    Article Title: Characterization of Reconstructed Human Epidermis in a Chemically-defined, Animal Origin-free Cell Culture System
    Article Snippet: .. Briefly, RNA from RHE was extracted using the “ReliaPrepTM miRNA Cell and Tissue Miniprep System” (Promega Cat#Z6111, Wisconsin, USA) and its concentration measured by NanoDrop 1,000 spectrophotometer (ThermoFisher Scientific, Massachusetts, USA). cDNA was then synthesized using “SuperScript III RNase H-Reverse Transcriptase Kit” (Thermo Fisher Scientific Cat18080093, Massachusetts, USA). .. SYBR Green method (Eurogentec, Liège, Belgium) and specifically designed primers (Eurogentec, Liège, Belgium; Millipore Sigma) listed in were used.

    Quantitative RT-PCR:

    Article Title: SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role
    Article Snippet: For RT-PCR analysis, total RNA was isolated from cells under standard culture conditions (un-confluent) using RNAiso Plus (Takara Bio, Shiga, Japan) in accordance with the manufacturer’s instructions. .. First-strand cDNA was synthesized from 1 μg of total RNA using a SuperScript III RNase H reverse transcriptase kit (Thermo Fisher Scientific). cDNA was then subjected to real-time RT-PCR using SYBR Premix Ex TaqTM II (Takara Bio) on a CFX96 Real-Time System (Bio-Rad Laboratories, Hercules, CA, USA). ..



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